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EDTA affects various stages of biofilm development. Biofilms were grown in inducing medium for 0 h, 4 h, 8 h, or 24 h. After this time, the medium was removed and replaced with fresh inducing medium containing various concentrations of EDTA (0 to 250 mM). After an additional 24 h of incubation, cell viability was measured by an XTT assay. Measurements were performed in triplicate under each condition, and error bars represent standard deviations.
