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. 2012 Nov;78(22):8015–8024. doi: 10.1128/AEM.02355-12

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Fig 5 — Heterologous expression of the β-carboline gene cluster in S. avermitilis SUKA22 and determination of the minimal gene cluster. (A) Schematic representation of the fragments used in this experiment. I, pLT437 containing genes from kse_70630 to kse_70700; II, pLT438 containing genes from kse_70630 to kse_70690; III, pLT439 containing genes from kse_70630 to kse_70670; IV, pLT440 containing genes from kse_70660 to kse_70700. The genes indicated by arrows are completely included in each fragment. (B) HPLC analyses (276 nm) of the metabolite profiles of S. avermitilis SUKA22 carrying pLT437 (I), pLT438 (II), pLT439 (III), pLT440 (IV), and pKU492Aaac(3)IV as an empty vector, respectively. JBIR-133 and kitasetaline are eluted at 26.7 and 28.0 min, respectively.