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Model for the B. anthracis amoebal life cycle illustrated with time-lapse phase-contrast micrographs. The following stages are shown: spore uptake within the first 2 h when cells were cocultured at 37°C (sp, spores) (frame i); germination and replication of B. anthracis with subsequent destruction of amoebas after ≈6 h (frame ii) (g, germinated spores); sporulation of B. anthracis with release of spores (r) from the mother cell after 12 h (frame iii).
