Table 2.
Primers used in this study
| Name | Sequence | Purpose |
|---|---|---|
| Qsynw1628for | TCTGGTCCATGTTGAGACGA | Q-PCR of synw1628 |
| Qsynw1628rev | TGACTGTGCAGCTCAAGACC | Q-PCR of synw1628 |
| Qsynw2127for | GACCAATCTTCTGGCTTCTCC | Q-PCR of synw2127 |
| Qsynw2127rev | GGGCTGGAGATCAATAGCAA | Q-PCR of synw2127 |
| Qsynw1709for | TGAAGATTGCAGACCGACAG | Q-PCR of synw0709 |
| Qsynw1709rev | AGTTGCGAAAGGCTGAAAAG | Q-PCR of synw0709 |
| Incsynw0635for | GTTGCTGCACCCTCCA | Inactivation of synw0635 |
| Incsynw0635rev | AGCAGCCCACAAGGATCA | Inactivation of synw0635 |
| Incsynw1628for | CAGCATCCTCACCGGTTT | Inactivation of WH8102 sodT |
| Incsynw1628rev | GGCACCCACCACCAGTAG | Inactivation of WH8102 sodT |
| Incsynw1626for | GTGTCGCGGCTGAAGCC | Inactivation of WH8102 sodN |
| Incsynw1626rev | CAGTTCCTCAGCTTTGGC | Inactivation of WH8102 sodN |
| Incsync0753for | AGCCATGAAGTCAGGAATGG | Inactivation of CC9311 sodT |
| Incsync0753rev | CTGGTCTTGGATTGCTCCAT | Inactivation of CC9311 sodT |
| IncSyncc9902_1528for | CCAGAAGATGACTCGCACCT | Inactivation of CC9902 sodT |
| IncSyncc9902_1528rev | CGATTGGCTTGAAAGACCTC | Inactivation of CC9902 sodT |
| IncSyncc9605_0871for | GCAGAGCATCGTCATCTTCA | Inactivation of CC9605 sodT |
| IncSyncc9605_0871rev | GATCGAGAGAGCACCTGTCC | Inactivation of CC9605 sodT |
| pMUTgibsonFor | CTGTTGCCCGCTTGGCGGGGCTGCTAGCGCTATATGCGTTGATG | Linearization of pMUT vectors with sodB sticky ends |
| pMUTgibsonRev | CCAACCTGGCTGCCGCCTGACCGCAAGAGGCCCGGCAGTA | Linearization of pMUT vectors with sodB sticky ends |
| Synwh7803_1742gibsonFor | CGCATATAGCGCTAGCAGCCCCGCCAAGCGGGCAACAG | Adding pMUT sticky ends to WH7803 sodB+ promoter sequence |
| Synwh7803_1742gibsonRev | TACTGCCGGGCCTCTTGCGGTCAGGCGGCAGCCAGGTTGG | Adding pMUT sticky ends to WH7803 sodB+ promoter sequence |
| VerSynw1626For | ATGCTGCGCTCGGCCCTCACCG | PCR verification of sodN::sodB |
| VerSynw1626Rev | GCCCTTGGATTGCCAGAACATGCC | PCR verification of sodN::sodB |
| Versynw1628 for | GGATCAGGGCTCGTTGCTGGTGA | PCR verification of sodT::sodB |
| Versynw1628 rev | GTGCAGCTCAAGACCGAAG | PCR verification of sodT::sodB |