Table 4.
[Ni2+] (pM) | pNi | Ni uptake rate with 5 nM Ni (zmol cell−1 min−1) |
---|---|---|
0.5 | 12.3 | 4.75 |
5 | 11.3 | 1.90 |
50 | 10.3 | 1.00 |
The ratios or fold changes of Ni uptake rates at 5 nM total Ni and the expression of the indicated genes were determined for semicontinuous cultures grown on NH4+ at the indicated [Ni2+] where pNi = −log10[Ni2+]. The values are shown as the ratios of the measured rates and gene transcript abundance for the indicated cultures. Ranges for triplicate Q-PCR measurements on duplicate cultures are shown.