Table 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Bacterial strains | ||
| R. eutropha | ||
| HF884 | ΔphaC1; mutant of H16 defective in PHB synthesis | 37 |
| HF950 | ΔphaC1 ΔhoxA; mutant of HF884 defective in lithoautotrophic growth | This study |
| HF952 | Mutant of HF884 with cphA6308 under the control of the PcbbL promoter integrated in the nor2 region of chromosome 2 | This study |
| E. coli | ||
| S17-1 | recA proA thi-1 hsdR; harbors the RP4 tra genes in the chromosome | 39 |
| Plasmids | ||
| pLO1 | Kmr sacB RP4 oriT ColE1 ori | 22 |
| pLO11 | Tcr RK2 ori Mob+; expression vector for Strep-tagged fusion proteins in R. eutropha | 38 |
| pCM62 | Tcr; bhr cloning vector | 26 |
| pSK::cphABsyn6308 | Derivative of pBluescript SK(−) carrying the genes cphA and cphB of Synechocystis sp. PCC6308 | 1 |
| pCH553 | pLO1 containing hoxA with 921-bp in-frame deletion | 23 |
| pCH700 | pLO1 containing a 2.6-kb EcoRV fragment with a 4.9-kb StuI deletion (ΔnorR2A2B2) | 34 |
| pCH1609 | 3.3-kb MspA1I fragment of pGE778 in pCH700 StuI | This study |
| pGE295 | Derivative of pVK101 carrying the hoxABCJ region of Alcaligenes sp. strain M50 | O. Lenz |
| pGE777 | 0.21-kb PCR fragment PcbbL sequence ligated to AflIII/NcoI-cut pLO11 | O. Lenz |
| pGE778 | pLO11 containing PcbbL-cphA6308 | This study |
| pGE779 | pLO11 containing PcbbL-cphA6308 and hoxABCJAlcaligenes sp. M50 | This study |