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Primer sequences for quantitative RT-PCR
| Gene | Primer direction | Primer sequence (5′ → 3′) |
|---|---|---|
| murF | Forward | GTTCCATACGCATACCAGTTAAGCT |
| Reverse | TGCGGTTGGTCATGAATTAGG | |
| pbp2 | Forward | ACGTCGTTAACAGAAACCAAGCA |
| Reverse | TGCGGTTGGTCATGAATTAGG | |
| vraR | Forward | TGAGTCGTCGCTTCTACACCAT |
| Reverse | ATTGCCAAAGCCCATGAGTT | |
| aaa | Forward | TTTAATCGTCGTGCTGAAATTGG |
| Reverse | TGCTGCCGCTGCGTTAT | |
| 16S rRNA gene | Forward | CAACGAGCGCAACCCTTAAG |
| Reverse | TTTGTCACCGGCAGTCAACTT |
