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Detection of the introduced cbxr gene in transformants. PCR products amplified from genomic DNA of transformants or the wild-type strain were directly sequenced. Sequencing chromatograms are shown for mutation site of the sdhB in the wild type and in the carboxin-resistant transformants. Any two overlapping peaks indicate the nucleotide from the endogenous sdhB and the transformed cbxr. Arrowheads indicate the mutated nucleotides.
