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Identification and characterization of the tHMGR gene-overexpressing strains. Amplification patterns were obtained with primers for the fusion gpd promoter-tHMGR gene fragment in genomic DNA isolated from different strains. Lane N, negative control; lane WT, wide-type strain; lane TR, the strain transformed with pJW; lane H, the tHMGR overexpressed strain; lane P, pJW-tHMGR as a positive control; lane M, DL 2000 DNA marker.
