Figure 1. VP24 key features. (A) Purified EBOV_1–233 VP24, SUDV_1–233 VP24 and RESTV_11–237 VP24 bind to purified STAT1_1–683 by ELISA. VP24s were coated onto the ELISA plate at 0.02 mg/ml for overnight incubation at room temperature. STAT1_1–683 was incubated on the plates, binding was detected with HRP-conjugated secondary antibody and the resulting O.D. was read at 450 nm. BSA was used as a negative control. (B and C) The overall shape of VP24 resembles a three-sided pyramid with Faces 1 and 3 as illustrated. A hydrophobic cavity in Face 1 and a polar cavity in Face 3 are indicated by arrows. A ribbon model of SUDV_1–233 VP24 illustrates the underlying secondary structural elements, while a surface representation of SUDV_1–233 VP24 indicates the sequence conservation. Navy indicates residues that are absolutely conserved among the ebola- and marburgviruses, while red indicates residues that are least conserved among these viruses. Face 2 is the least conserved of the three faces and is not illustrated. Sequence identity is calculated using Homolmapper.³⁷ The accession codes for SUDV_1–233, SUDV_11–233 and RESTV_11–237 are 3VNE, 3VNF and 4D9O, respectively.