Fig 1.

Pseudohypha formation of an αAAα diploid in response to nitrogen limitation. (A) The αAAα diploid strain MMRL1368 produced pseudohyphae on the edge of colonies when glucose, galactose, or sucrose was used as the fermentable carbon source. However, when maltose was used as the carbon source, the strain did not form pseudohyphae. (B) Differential interference contrast (DIC) image of pseudohyphae of MMRL1368 displays branched pseudohyphae without the clamp connections observed during hypha formation. (C) Scanning electron microscopic images show details of pseudohyphae. Scale = 5 μm.