Fig 3.

Images of [¹⁴C]AIB in colonies of WT and mutant N. crassa strains over time. (A) Images of photon emissions were captured at 4, 8, 16, 32, and 64 h from the WT, ΔPrm-1, and Δso colonies where [¹⁴C]AIB was added to the inoculation plugs (I*) prior to growth of the colony. Unlabeled sucrose resource plugs (R) were placed ∼4 cm from the inoculation plugs. (B) Photon emissions captured at 4, 8, 16, 32, and 64 h from the WT, ΔPrm-1, and Δso colonies where [¹⁴C]AIB had been added to a sucrose resource plug (R*) prior to growth, but the inoculum plug (I) remained unlabeled. Resource plugs were placed ∼4 cm from the inoculation plugs.