Fig 4.

Double mutant analyses in A. nidulans. (A) WT (TNJ36), ΔAnricA (TNJ21), ΔAnsfgA (TNJ57), ΔAnsfgA ΔAnricA (TNJ58) ΔAnflbA (TNJ42), and ΔAnflbA ΔAnricA (TNJ49) strains grown on solid MMG for 5 days at 37°C. (B) WT (TNJ36), ΔricA (TNJ21), ΔrgsA (TNJ61), ΔricA ΔrgsA (TNJ62), ΔrgsB (TNJ63), ΔricA ΔrgsB (TNJ64), ΔrgsC (TNJ65), and ΔricA ΔrgsC (TNJ66) were inoculated on MMG and incubated at 37°C for 5 days. (C) Germination of WT (TNJ36), ΔrgsA (TNJ61), and ΔricA ΔrgsA (TNJ62) conidia in the absence of an external carbon source. Photographs were taken after inoculating 1 × 10⁶ conidia into liquid MM without an external C source and cultured for 16 h at 37°C and 220 rpm. Bar, 25 μm.