Table 1.
Estimation of IC50s for three selection drugs tested on S. salmonicida cellsa
Selection drug | IC50 (μg/ml) | Suggested concn for use in selection (μg/ml) | % growth inhibition at selection concn |
---|---|---|---|
Puromycin | 18 | 50 | 96 |
Blasticidin S | 7 | 15 | 92 |
G418 | 49 | 150 | 94 |
Hygromycin B | 120 | 300 | 88 |
Paromomycin | 180 | 350 | 84 |
The cells were treated with the indicated drugs and counted manually at given time points. This table shows the results from the fifth day of drug treatment. The data were used for plotting the percent growth inhibition against the concentrations tested, from which the IC50s were estimated.