Fig 3.

Levels of camp gene expression induced by infection with S. Typhi and S. Typhimurium. Day 12 MDMs were infected in duplicate with S. Typhi (A) or S. Typhimurium (B) at an MOI of 1:10 (MDM/bacteria) for 2.5, 10, or 24 h prior to collection of eukaryotic RNA. MDMs were also treated with 100 nM 1,25D3 for 24 h as a positive control for the induction of camp gene expression. The graphs represent the pooled results from two donors; however, these results have been confirmed using MDMs from additional donors infected with select Salmonella strains (n = 3). Statistical significance was determined by Student's t test comparing RCNs from a 24-h uninfected sample to a 24-h 1,25D3-stimulated sample. ***, P < 0.00005.