Fig 2.
EspZ decreases the rate of death of infected enterocytes. C2BBE cells grown on glass coverslips were treated with medium alone or infected with EPEC, the ΔespZ mutant, or the single-copy complemented cis-espZ strain for 1, 2, and 4 h and then stained with an ethidium homodimer 15 min prior to imaging. Representative data from three independent experiments are shown. Percentages represent averages from 9 fields.