Fig 3.
FAK phosphorylation is not involved in espZ-dependent intestinal epithelial cell survival. Mean propidium iodide uptake values (six technical replicates) are shown for T84 (A), C2BBE (B), and HeLa (C) cells infected with WT EPEC or the ΔespZ or cis-espZ strain in the presence of the FAK inhibitor PF-573228 (50 μM) or DMSO as a vehicle control. After 4 h, cells were treated with propidium iodide (2 μg/ml), and fluorescence was measured. To obtain a measurement of maximum fluorescence (100% death), control samples were treated with methanol prior to the addition of PI. *, P < 0.05; **, P < 0.01 (determined by Student's t test). Each experiment was repeated at least 2 times (n = 3 for panel A, n = 2 for panel B, and n = 3 for panel C).