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EspZ inhibits cytochrome c release in EPEC-infected cells. C2BBE cells were treated with medium alone or infected for 4 h with WT EPEC or the ΔespZ or cis-espZ strain. Host cell cytosolic and mitochondrial fractions were separated and analyzed for the abundance of cytochrome c by Western blotting. Blots were also probed for Cox4 as a control marker for the mitochondrial fractions. The blot shown is representative of three independent experiments.
