Fig 1.

CCL2 and CXCL2 responses to Pneumocystis (Pc) in primary alveolar epithelial cells are dependent on MyD88. Primary mouse AECs were isolated and cultured from C57BL/6 mice (WT) and C57BL/6 Myd88^−/− mice. Confluent monolayers were treated with freshly isolated murine Pneumocystis at cyst-to-AEC ratios of 1:1 and 3:1. AECs were also treated with either medium alone as a negative control (NT) or TNF-α (10 ng/ml) as a non-MyD88-dependent positive control. Cells were also treated with LPS (10 ng/ml) and Pam3CSK4 (0.5 μg/ml). CCL2 levels in the supernatants at 6 h (A) and 12 h (B) were measured by ELISA (*, P < 0.05 compared to the same treatment in WT). (C) CXCL2 levels in supernatants were measured at 6 h and 12 h (*, P < 0.05 compared to all other groups at each time point). Results are shown as the fold increase relative to medium-only treatment. Bars represent means ± SEMs (n = 3) from a representative experiment that was repeated four times.