Fig 2.

MyD88 is required for Pneumocystis-stimulated CCL2 mRNA expression in AECs. Primary murine pneumocytes were isolated from C57BL/6 WT and Myd88^−/− mice. Confluent monolayers were exposed to freshly isolated murine Pneumocystis at a Pneumocystis-to-AEC ratio of 3:1. AECs were also treated with medium alone as a negative control (WT or MyD88^−/−). (A) RNA was collected after 6 h of treatment and analyzed by RPA. KO, knockout. (B) MCP-1 mRNA levels were quantified and normalized to the levels of the housekeeping gene mL32.