Fig 3.

CCL2 response of Pneumocystis-stimulated AECs is independent of TRIF. Primary murine pneumocytes were isolated from C57BL/6 WT and TRIF^−/− mice. Confluent monolayers were exposed to freshly isolated murine Pneumocystis at cyst-to-AEC ratios of 1:1 and 3:1. AECs were also treated with either medium alone (negative control [NT]) or TNF-α (10 ng/ml). CCL2 levels in the supernatants at 6 h were measured by ELISA (P > 0.05). Bars represent means ± SEs (n = 3) from a representative experiment that was repeated twice.