Fig 6.

CCL2 production by Pneumocystis-stimulated AECs is independent of TLR2 and/or TLR4. Primary murine AECs were isolated from WT and MyD88^−/−, TLR2^−/−, TLR4^−/−, and TLR2/4 double-knockout mice all on the C57BL/6 background. Confluent monolayers were exposed to Pneumocystis (at cyst-to-AECs ratios of 1:1 and 3:1), TNF-α (10 ng/ml), LPS (10 ng/ml), or Pam3CSK4 (0.5 μg/ml). At 6 h poststimulation, cell supernatants were collected and CCL2 chemokine responses were tested by ELISA. Bars represent means ± SEs (n = 3) from a representative experiment that was repeated three times (*, P < 0.05 compared to the same treatment in WT).