Fig 4.

Downregulated proteins in FN-bound amoeboid cells compared with trophozoites. (A) Magnified regions of the 2-DE maps showing the 31 downregulated proteins in the FN-binding proteome. Protein spots with a lower abundance in the amoeboid stage are numbered and indicated by arrows. (B) Each bar represents the relative abundance expressed as the normalized spot volume calculated by using Phoretix 2D analysis software. Details of their information are shown in Table 2. (C) Functional categories of the downregulated proteins identified by MALDI-TOF PMF classified according to the Gene Ontology index (http://www.geneontology.org/).