Fig 6.

qPCR analysis validating the comparative transcriptome and proteome data. mRNA expression levels of eight genes with differential expression in our FN-binding transcriptome and proteome results were confirmed by qPCR analysis compared with the trophozoite stage. Data are expressed as log fold changes (log base 2) ± standard deviations of the means from three independent experiments. Transcripts selected for qPCR validation were tryparedoxin peroxidase (TXNPx) (TVAG_455310 and TVAG_038090), iron superoxide dismutase (SOD) (TVAG_039980 and TVAG_120340), thioredoxin reductase (TrxR) (TVAG_474980), a flavodoxin family protein (TVAG_216360), GAPDH (TVAG_146910), and malate dehydrogenase (MDH) (TVAG_171090).