Table 7.
Relationship between fold changes of mRNA transcripts and corresponding protein concentrations in monocyte supernatants after 3 h of incubationa
| Cytokine | Gene expression (fold change) |
Concn in culture supernatants (ng/ml)b |
% reductionc | |||||
|---|---|---|---|---|---|---|---|---|
| N. meningitidis vs control | N. meningitidis + IL-10 vs control | IL-10 vs control | N. meningitidis | N. meningitidis + IL-10 | IL-10 | Control | ||
| IL-1β | 1.5 | 1.3 | −1.3 | 1.8 ± 0.3 | 0.13 ± 0.01 | N/A | 0.008 ± 0.002 | 93%* |
| IL-6 | 6.4 | 2.2 | NC | 7.9 ± 0.6 | 3.20 ± 0.4 | 0.09 ± 0.02 | 0.24 ± 0.1 | 49%* |
| IL-8 | 1.6 | 1.2 | −1.3 | 14.9 ± 1.6 | 6.5 ± 0.9 | 0.4±.05 | 1.4 ± 0.4 | 58%* |
| TNF-α | 6.2 | 2.0 | −2.3 | 10.0 ± 0.7 | 2.3 ± 0.2 | 0.04 ± 0.008 | 0.1 ± 0.01 | 36%* |
| MIP-α | 2.5 | 2.1 | −2.2 | 12.5 ± 1.6 | 6.6 ± 0.8 | 0.07 ± 0.02 | 0.2 ± 0.04 | 41% |
| MIP-β | 3.3 | 2.2 | −2.1 | 10.8 ± 1.5 | 5.6 ± 1.1 | 0.3 ± 0.1 | 0.9 ± 0.2 | 39.8%* |
a
NC, no change; N/A, below detection limit.
b
Values are means ± standard errors of the means (five samples per group) for cytokines secreted by monocytes treated with each inducer. Means were compared with paired t tests.
c
Reduction in N. meningitidis plus IL-10 compared with N. meningitidis alone. Asterisk indicates significance (P < 0.01 after Bonferroni correction for the number of samples) between N. meningitidis and N. meningitidis in combination with IL-10.