Fig 10.
GST-OmpA inhibits A. phagocytophilum binding to sLex-capped PSGL-1. A. phagocytophilum DC organisms were added to PSGL-1 CHO cells that had been incubated with GST-OmpA, GST alone, CSLEX1, or mouse IgM, and incubation continued in the presence of recombinant protein or antibody for 1 h. After removal of unbound bacteria, the numbers of A. phagocytophilum DC organisms bound to PSGL-1 CHO cells were determined using indirect immunofluorescence microscopy. Results shown are representative of two independent experiments with similar results. Statistically significant (***, P < 0.001) values are indicated.