Fig 5.
Regulation of the expression of integration and excision genes of MGIs and SXT/R391 ICEs. (A) Impact of protein 9 on the expression of intMGI. The expression of intMGI was measured by quantitative PCR upon induction of the SOS response (+MC) in AD72, AD130, and AD167 or overexpression of cds9 from p9 in AD167 p9 (+Ara). One-way analysis of variance with a Dunnett posttest was used to compare the means of relative expression of intMGI against the strain devoid of an ICE. The confidence interval for the comparisons was P < 0.01 (**). ns, nonsignificant. (B) Impact of SetCD on intMGI and rdfM expression. The expression of yicC, intMGI, and rdfM was measured upon induction of the SOS response (+MC) in AD72 and AD133 or upon overexpression of setCD from pGG2B (+Ara) in AD132.