Table 2.
DNA sequences of the primers used in this study
| Primer name | Nucleotide sequence (5′ to 3′) | Use in the study |
|---|---|---|
| AD4-V-F | TAGCAGTGAGGAAGCAAACGATG | Amplification of cds9MGIVvuTai1 |
| AD4-R1 | TTATCCACGGCCATAAGCAGC | Amplification of cds9MGIVvuTai1 |
| AD5-F | GCCGTGGATAAACCATCAGCA | Amplification of cds8MGIVvuTai1 |
| AD5-V-R1 | TTAGTCATCCAAAATACTGCCTTT | Amplification of cds8MGIVvuTai1 |
| AD5-A-R1 | TTAGTCATCCAAGATGCTGCCTTT | Amplification of cds9-8MGIVflInd1 |
| AD4-A-F | TAGCCGATTAGTACTGGCAAACTCC | Amplification of cds9-8MGIVflInd1 |
| AD11-WF | CAGCCCACGGCACGCGCACCAATACGAATGGAACGTGTGTAGGCTGGAGCTGCTTCG | Deletion of cds9 in MGIVflInd1 |
| AD11-WR | ATGAACCCAACTACACAATCATCCACCACATCAACAATTCCGGGGATCCGTCGACC | Deletion of cds9 in MGIVflInd1 |
| AD13-WF | AGTGCTAACGATTGGGATAGAGAATGGATACAGCAGGTGTAGGCTGGAGCTGCTTCG | Deletion of cds4 in MGIVflInd1 |
| AD13-WR | CAGCGCCCTGTGAGGGGTTACTCTTTTTTCAGGCCTATTCCGGGGATCCGTCGACC | Deletion of cds4 in MGIVflInd1 |
| attPAD-L1 | TCGGCTTTGCTGTATGCAATA | Amplification of attPMGI, 1st round |
| attPAD-R1-AC | TCTGCCATAGCAACAGCAAT | Amplification of attPMGI, 1st round |
| attPAD-L2 | GAGTTTTCCCATGTTTACTCCATA | Amplification of attPMGI, 2nd round |
| attPAD-R2-AC | GTGACAGCTTTGCCTGCTT | Amplification of attPMGI, 2nd round |
| Gene8-WF | TATGCCTAGCAACATGCCAAAATTACCAGCTGGTTTGTGTAGGCTGGAGCTGCTTCG | Deletion of cds8 in MGIVflInd1 |
| Gene8-WR | TGACTTTGCGCTGTGGTCGGTTGCCATCGGGGATTAATTCCGGGGATCCGTCGACC | Deletion of cds8 in MGIVflInd1 |
| Q-PCR-1F | AAGTGACAAACTCCGCCATC | Amplification of attB in E. coli |
| Q-PCR-1R | GCACGCAAAACAGAATTGAA | Amplification of attB in E. coli |
| Q-PCR-2F | GAAAACGGCAAGCTGAAAAC | Amplification of rph in E. coli |
| Q-PCR-2R | GTCCCCTGCACTTCAATGAT | Amplification of rph in E. coli |
| RTgene9-F b | TTATCCACGGCCATAAGCAG | Amplification of cds9MGIVflInd1 |
| RTgene9-R b | AGGTAAAGGCCAACTCAGGCTT | Amplification of cds9MGIVflInd1 |
| RTintVf-F | CCGTATCGGGTTTACACCAA | Amplification of intMGIVflInd1 |
| RTintVf-R | TTATCGCATGTCGAAACAGC | Amplification of intMGIVflInd1 |
| RTrpoZcoli-F | GCTCGTCAGATGCAGGTAGG | Amplification of rpoZ |
| RTrpoZcoli-R | GCTTGTAATTCAGCGGCTTC | Amplification of rpoZ |
| RTyicC-F | GAGTGACGAAGGGGAAATCA | Amplification of yicC |
| RTyicC-R | GCTTTCAGTGCCTGACCTTC | Amplification of yicC |