Table 2.
Summary of results of plaque assays with P. aeruginosa PA14 CRISPR/cas mutantsa
Phage | Genotypeb |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|
WT | ΔCR/cas | ΔCR1 | ΔCR2 | Δcas1 | Δcas3 | Δcsy1 | Δcsy2 | Δcsy3 | Δcsy4 | |
JBD18 | − | + | − | − | − | + | + | + | + | + |
JBD25 | − | + | + | − | − | + | + | + | + | + |
JBD67 | − | + | − | − | − | + | + | + | + | + |
WT DMS3 | + | + | + | + | + | + | + | + | + | + |
DMS3 C253G | - | + | - | + | - | + | + | + | + | + |
DMS3 T255C | - | + | - | + | - | + | + | + | + | + |
DMS3100% | − | + | − | + | − | + | + | + | + | + |
Plaque assays conducted with the indicated phages used to infect lawns of WT or various mutant strains.
+, formation of plaques (EOP, 1 relative to ΔCR/cas); -, reduced plaquing efficiency with a >104-fold reduction; −, reduced plaquing efficiency with a >106-fold reduction.