Fig 2.

Interaction of ParB1 with segS-type centromeres of chromosome I. All three chromosomal-type centromeres, segS1 (A), segS2 (B), and segS3 (C), were PCR amplified, and approximately 300-bp PCR products of each element were labeled with [γ-³²P]ATP and incubated with different concentrations of recombinant ParB1. For competition with 400 bp nonspecific DNA, the segS elements were incubated with ParB1 and chased with 1:1 (lane 1), 1:5 (lane 2), 1:10 (lane 3), 1:20 (lane 4), 1:50 (lane 5), and 1:100 (lane 6) molar ratios of 250 bp of nonspecific dsDNA (Non-spDNA). Products were separated on 5% native PAGE gels and dried, and autoradiograms were developed. Experiments were repeated two times, and reproducible data from a representative experiment are shown.