Fig 3.

Activity characterization of recombinant ParA1. (A) The DNA binding activity of purified ParA1 was checked with 400 bp PCR-amplified nonspecific DNA and increasing concentrations of protein, and products were analyzed on a 1.2% agarose gel. S, supernatant. (B) The ATPase activity of ParA1 was measured with cold ATP, and the release of inorganic phosphate was estimated as a function of the ParA1 concentration. (C) Similarly, the ATPase activities of ParA1, ParB1, and ParA1 plus ParB1 (A1B1) incubated with aberrant parS (Dp1+A1B1), segS1 (S1+A1B1), segS2 (S2+A1B1), and segS3 (S3+A1B1) of chromosome I were measured as described in Materials and Methods, and the release of inorganic phosphorous was estimated.