Fig 4.

Sedimentation analysis of ParA1 in the presence of its cognate putative chromosome I partitioning components. Recombinant ParA1 was incubated with segS1 (S1), segS2 (S2), segS3 (S3), ParB1, and ATP in different combinations. (A) Mixtures were centrifuged at 22,000 × g for 30 min, and both supernatant (S) and pellet (P) fractions were analyzed by SDS-PAGE. (B) The gel was stained with silver nitrate, and the intensity of the protein band corresponding to ParA1 was estimated by densitometric scanning of the respective gel. Each experiment was repeated three times, and results from one reproducible experiment are shown in panel A and with standard deviations in panel B.