Fig 5.

Light scattering studies of ParA1 incubated with ParB1 and segS elements in the presence and absence of ATP. Recombinant purified ParA1 (ParA1) and ParB1 (ParB1) were taken alone and together in different combinations with segS1 (A), segS2 (B), and segS3 (C), and ATP and light scattering spectra were recorded as a function of time. For experiments where both ParA1 and ParB1 were incubated with segS elements, ParB1 was preincubated with segS before ParA1 was added. For the ATP effect, the ATP was added last in any given combination. Experiments were repeated at least three times independently. Data presented here are the means of values obtained with 10 shots of 10 s each at every single time point from a representative experiment.