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. 2012 Nov;194(21):5769–5782. doi: 10.1128/JB.01264-12

Fig 6.

Fig 6

H-NS represses upaH expression. (A) β-Galactosidase assay of upaH::lacZ-zeo reporter fusions in strain CFT073, isogenic hns deletion mutant, complemented mutant, and complement vector control. (B) Electrophoretic band shift of the amplified 250-bp upaH promoter and the bla promoter from TaqI-SspI-digested pBR322 DNA in the presence of increasing concentrations of H-NS (0.05, 0.1, 0.2, 0.5, 1, and 2 μM). The pBR322 fragments not containing the bla-promoter (313/315 bp and 475 bp) were not shifted by H-NS. (C) Curvature-propensity plot of the sequence (250 bp) upstream of the upaH gene from CFT073(PupaHCFT073) and M369(PupaHM369) showing the predicted regions of curved DNA. (D) Curved DNA PAGE gel of the amplified 250-bp promoters PupaHCFT073 and PupaHM369.