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Rip1 is sensitive to C-terminal epitope tagging. (A and B) Growth assay at 30°C (A) as well as bc1 complex activity assay and SDS-PAGE immunoblot of isolated mitochondria (B), from WT and rip1Δ cultures expressing vector control (vec) or YEp or YCp RIP1-Myc or YCp RIP1-3HA plasmid. (C) BN-PAGE immunoblot of digitonin-solubilized mitochondria isolated from the same cultures as in panels A and B.
