Fig 3.

LAPTM5 expression elicits the downmodulation of the pre-BCR in the absence of BLNK. (A and B) BKO/Ton-LAPTM5 cells were cultured with doxycycline (Dox) for the indicated time periods or stimulated with pervanadate (PV) for 5 min and subjected to immunoblot analysis for the expressions of LATPM5 and Lyn (A) and Syk, ERK, and their phosphorylated forms (B). Note that in the sequential blotting, the bands of Syk and ERK in pervanadate-stimulated cells did not show up well due to strong signals for the phosphorylated proteins in the first blotting. (C) BKO/Ton-LAPTM5 cells were cultured with doxycycline (filled histograms) or control H₂O (open histograms) for 24 h (top) or for the indicated time periods (bottom) and analyzed for the surface expression of pre-BCR and CD19 as described in the legend to Fig. 1A (means ± standard errors of the means; n = 4 for each time point) (∗∗, P < 0.01). Data shown in panels A to C are representative of three independent experiments.