Fig 5.

LAPTM5 promotes lysosomal degradation of the intracellular pool of pre-BCR. (A) BKO/BASH-ERtm cells infected with an hCD8-expressing retroviral vector carrying Laptm5 shRNA (Laptm5sh-1) or an irrelevant control shRNA were cultured for 24 h with 4-OHT or control ethanol, as described in the legend to Fig. 2D and E, and subjected to immunoblot analysis for the detection of the indicated proteins. Open and filled arrowheads indicate mature and immature forms of μH chains, respectively. Data shown are representative of data from three independent experiments. (B) BKO/Ton-LAPTM5 cells were cultured for 24 h with doxycycline or control H₂O and subjected to immunoblot analysis for the detection of the indicated proteins. (C) BKO/Ton-LAPTM5 cells were cultured with doxycycline or control H₂O for 12 h, and bafilomycin or vehicle (DMSO) was included in the culture medium during the last 4-h period, followed by immunoblot analysis, as described above for panel B. Data shown in panels B and C are representative of data from five independent experiments.