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Knocking down KLF4 has no long-term effect on differentiated neurons. Representative images of coronal brain sections, which were electroporated at E14.5 and examined at P3, are shown. A pCAG-EGFP plasmid was coelectroporated to label transfected cells. Sections were counterstained with Hoechst (blue signal). Arrowhead marks axonal bundles that crossed the midline. Images in panels B and D are higher-magnification views of boxed regions in panels A and C, respectively. Scale bar, 50 μm.
