Table 3.

Effects of coexpression of L1 and PABPs on cell sensitivity to puromycin

L1 construct	Cell viability under puromycin selection relative to control levela
	pcDNA-PABPN1	pcDNA-PABPC1
ORFeus-Hsb	0.62 ± 0.07	0.62 ± 0.08
ORFeus-Hs EN−c	0.61 ± 0.07	0.63 ± 0.07
ORFeus-Hs RT−d	0.60 ± 0.03	0.52 ± 0.04
ORFeus-Hs EN− RT−e	0.60 ± 0.06	0.55 ± 0.08

^aThe value for coexpression of L1 and empty pcDNA was set at 1. Data are means ± standard errors for three independent experiments.

^bWild-type ORFeus-Hs cloned into pCEP-puro.

^cORFeus-Hs H230A mutant cloned into pCEP-puro.

^dORFeus-Hs D702A mutant cloned into pCEP-puro.

^eORFeus-Hs H230A D702A mutant cloned into pCEP-puro.