Table 2.
Summary of genetic interactions involving dcd1Δa
| Allele | Function | Treatment |
||||
|---|---|---|---|---|---|---|
| Untreated | UV | HU | CPT | MMS | ||
| brc1Δ | BRCT domain protein | Yes | YES | YES | YES | YES |
| rad3Δ | ATR checkpoint kinase | SL | ||||
| chk1Δ | DNA damage checkpoint kinase | YES | YES | YES | YES | YES |
| cds1Δ | DNA replication checkpoint kinase | SL | ||||
| cds1.T8A | DNA replication checkpoint kinase mutant | Yes | YES | YES | YES | YES |
| mrc1Δ | Mediator of replication checkpoint 1 | SL | ||||
| rad1Δ | Checkpoint clamp complex protein | SL | ||||
| ddb1Δ | Damaged DNA binding protein | SL | ||||
| ssb3Δ | DNA replication factor A subunit | YES | YES | YES | YES | YES |
| swi1Δ | Replication fork protection complex subunit | YES | YES | YES | YES | YES |
| nse5Δ | Smc5-6 complex non-SMC subunit | YES | YES | YES | YES | YES |
| mus81Δ | Holliday junction resolvase subunit | YES | YES | YES | YES | YES |
| rhp51Δ | RecA family recombinase | YES | YES | YES | YES | YES |
| ctp1Δ | CtIP-related endonuclease | Yes | YES | YES | YES | YES |
a
Double mutants were assessed for growth in the absence or presence of specified genotoxins. YES, strong negative interaction; Yes, negative interaction; SL, synthetically lethal.