Fig. 3.

Effect of PPADS on hindlimb vasodilation evoked by HDA stimulation. a Pooled hindlimb vascular conductance (HVC) curves constructed from responses generated by stimulation of the HDA (150–200 μA, 0.6 ms, 100 Hz for 5 s) during control and after PPADS (5 mM; Sigma Aldrich, St Louis—USA) in the nucleus tractus solitarii (NTS). Blockade of P2 receptors with bilateral microinjections of PPADS into the NTS reduced the elicited vasodilation compared to control. b Group data (N = 4) showing reduction of maximum responses generated by hypothalamic stimulation after antagonism of P2 receptors with PPADS (PPADS NTS). ***P < 0.001