Fig 1.

LY294002 treatment decreases invasion and migration of melanoma cell lines. (A) Skmel28 invasive melanoma cells were treated with 20 μM LY294002. After 24 h, the number of LY294002-treated invading cells was counted and compared to the control (DMSO). Columns represent means from three experiments; bars indicate SD; *, P < 0.05. (B to D) LY294002 treatment decreases the wound-healing ability of the SKmel28 (B), B16 (C), and 501mel (D) melanoma cell lines in a scratch/wound assay. (E) Proliferation MTT assay on Skmel28 cells over 48 h in the presence or absence of PI3K inhibitors. Values represent means of optical density values measured at 560 nm ± standard deviations (SD).