Fig 5.

(A) Scheme representing the WT Brn-2 promoter reporter construct with the Lef/Tcf site in gray, the two putative Pax3 binding element underlined, and the mutated bases represented in italics. (B) Luciferase assay was performed on 501mel cells to confirm the loss of responsiveness of the Brn-2 promoter mutated for the Pax3 binding site after transfection of Pax3 or an empty vector (Ctl). Values reported are means from 2 independent experiments performed in triplicate; error bars represent SD.