Fig 1.

FUN30 does not affect interchromosomal gene conversion, break-induced replication, or single-strand annealing. (A) Viability of ectopic recombination strain YJK17 and its fun30Δ derivative on YEP-galactose plates relative to YEPD plates (see Materials and Methods). (B) Quantification of gene conversion kinetics based on Southern blot analysis. (C) Viability in BIR strain JRL346. (D) Viability in the SSA/BIR strains YMV80 and YMV45. (E) Microcolony assay of YMV80 strains harboring wild-type FUN30 or fun30 ATPase-defective mutant on plasmids. Data are means and standard errors of the means (SEM) from at least three independent experiments.