Fig 6.

Fun30 is recruited to a DSB in vivo and has lower affinity for γ-H2AX than for unphosphorylated H2A. (A) Chromatin immunoprecipitation of Fun30 modified by a FLAG epitope at its C terminus, after induction of an unrepaired DSB in strain JKM179. The increase of IP signal relative to input DNA is shown. Error bars reflect ranges from two independent experiments. (B) Purified-FLAG-tagged Fun30 was incubated with immobilized nucleosome arrays at different salt concentrations. (C) Anti-γ-H2AX antibody recognizes the phosphomimetic form of histone H2A.