Fig 8.
FUN30 is required for adaptation to a single, irreparable DNA DSB. (A) Single cells of wild-type, fun30Δ, and tid1Δ strains were plated on YEP-Gal plates and monitored at 8 h and 24 h. The percentage of cells adapted at any time point was calculated from the number of cells that progressed beyond the two-cell stage. Data are means and SEM from at least three independent experiments. (B) Wild-type and fun30Δ strains were stained with DAPI 24 h after galactose induction. The percentages of 150 G2/M-arrested cells that had a single nucleus are provided. (C) chk1Δ but not tel1Δ suppressed the adaptation defect of fun30Δ. (D) fun30Δ and tid1Δ prolong RAD53 hyperphosphorylation. Cells of the indicated strains were collected after galactose induction at the time points indicated. Proteins were extracted, and Western blotting was carried out using anti-Rad53 antibody. The arrows indicate the phosphorylated and unphosphorylated forms of Rad53.