Table 2.
Resection rates for mutants
| Strain genotype | Rate of DNA resection (kb/h)a |
|---|---|
| Wild type | 4.0 ± 0.7b |
| fun30Δ | 1.2 ± 0.1c |
| sgs1Δ | 3.0 ± 0.2b |
| sgs1Δ fun30Δ | 1.8 ± 0.7b |
| exo1Δ | 2.1 ± 0.4c |
| fun30Δ exo1Δ | 1.2 ± 0.2b |
| H2A-S129A | 6.8 |
| fun30Δ H2A-S129A | 2.4 |
a
5′-to-3′ resection rates from an HO-induced unrepaired DSB in strain JKM179 and its derivatives were measured as described in the legend to Fig. 3 and in Materials and Methods. Resection rates were calculated from qPCR values as described in the text.
b
Average of three time courses (± standard deviation).
c
Average of two time courses (± range).