Fig 1.

Neutrophil trafficking to inflamed peritoneum is impaired in KI mice. (A) Dot plots of WT and KI peritoneal cells at 4 h after intraperitoneal thioglycolate injection. SSC, side light scatter. (B) Total cell counts (filled symbols) of peritoneal exudates were obtained by flow cytometry. Neutrophil counts (open symbols) were calculated by multiplying the percentage of Gr-1⁺ CD11b⁺ cells by total cell counts. Data are means ± SEMs. (C) Surface expression of CXCR2, CXCR4, and C5aR on challenged peritoneal neutrophils. For intracellular CXCR2 (inCXCR2) expression, cells were fixed, permeabilized, and then stained for CXCR2. Since surface CXCR2 was not blocked prior to intracellular staining, intracellular CXCR2 represents a total CXCR2 level in the cell. (D) Surface CXCR2 expression on peritoneal KI monocytes was similar to that on WT cells. ISO, cells immunostained with isotype controls.