Fig 6.

The phenotype associated with KI mice is neutrophil intrinsic. CD45.1 WT mice were irradiated and received WT CD45.2 or KI CD45.2 bone marrow cells. Data shown are from two independent adoptive transfers. (A) Total cell and neutrophil counts of peritoneal exudates from thioglycolate-challenged CD45.1/CD45.2 chimeras at 4 h postchallenge. Data are means ± SEMs. PECs, peritoneal cells. (B) Blood neutrophil counts of chimeric thioglycolate-challenged mice. Data are means ± SEMs. (C) Percentages of Gr-1⁺ CD11b⁺ bone marrow cells from naïve chimeric mice are shown. Data are means ± SEMs. (D) Dot plots and percentages of three populations, promyelocytes/myelocytes (P/M), immature neutrophils (ImN), and mature neutrophils (MaN), within the total Gr-1⁺ CD11b⁺ bone marrow cells from naïve and challenged chimeric mice. Data are means ± SEMs. (E) Expression of CXCR2 and CXCR4 on bone marrow and blood neutrophils from naïve and challenged mice to which cells were adoptively transferred. ISO, neutrophils immunostained with isotype controls.