Fig 1.

Interlineage recombination in type-2 HAstVs. Lineage 2a (accession no. AB000289), 2b (L13745), 2c (EF138827), and 2d (JX087964) HAstVs were plotted against the recombinant strain NOR/1993/NORAS1128 (AB000290). Sequences were analyzed with Simplot (14) using a window size of 200 and step size of 20 with gap strip off and Hamming correction on. The recombination breakpoint is shown as a vertical dashed line. The genome organization of HAstV from the 3′ end of ORF1b to the poly(A) tail is also shown. The HVR region is in gray. The locations of the diagnostic regions D5′ and D3′ in the ORF2 are shown. Phylogenetic trees were constructed using the D5′ and D3′ regions. The D5′ tree was constructed using an ∼350-nt ORF2 fragment of a selection of 38 sequences of type 2 HAstV strains retrieved from the databases. The D3′ tree was constructed using an ∼200-nt ORF2 fragment of 21 sequences. For strains in boldface, the full-length ORF2 is available. The trees were generated by the neighbor-joining method and the Kimura 2-parameter distance correction, with 1,000 bootstrap replicates, using the software Mega 5 (22). Bootstrap values lower than 95% are not shown.